Gram staining

Dye solutions:

Solution A: Carbol gentian violet/crystal violet

Solution B: Lugol’s solution

Solution C: Ethanol (95%, methylated spirits)

Solution D: Safranin



1. Production of dry specimen

2. Allow Solution A to act for 90 seconds

3. Rinse with water

4. Allow Solution B to act for 60 seconds

5. Rinse with water

6. Sluice with ethanol (Solution C)

7. Rinse with water

8. Allow Solution D to act for 60 seconds

9. Rinse with water

10. Dry