Crystal violet staining

Dye solutions:

Crystal violet solution 0.2% dilution in distilled water (Solution A for gram staining 1:5 dilution)



1. Production of dry specimen

2. Apply several drops of dye solution to the specimen and spread carefully

3. Wash off dye solution under water

4. Dry the lower side of the slide only and place a cover glass on the film of water on the top side with the dyed dry specimen