Neisser staining

Dye solutions:

Solution A: Methylene blue in glacial acetic acid and ethanol

Solution B: Crystal violet in ethanol

Solution C: Chrysoidine Y



1. Production of dry specimen

2. Mix Solution A and Solution B in 2:1 ratio; allow to act on the specimen for 25 seconds

3. Dab dry with absorbent paper towel

4. Allow Solution C to act for 45 seconds

5. Rinse with water

6. Dry